IGROV1-specific miRNA–mRNA interactions. (A,B) RT-qPCR quantification of miR-141-3p (A) and miR-200c-3p (B) in DU145 and derived TUT4/7 cKOs. Number of biological replicates (n) for WT = 4. TUT4/7 cKO #1 represents one independent clone (n = 4) and TUT4/7 cKO #2 (n = 3) represents the other. P-values are denoted by p and are determined by Student's t-test. (C,D) RT-qPCR quantification of miR-141-3p (C) and miR-200c-3p (D) in IGROV1 and derived TUT4/7 cKOs. Number of biological replicates (n) for WT = 4. TUT4/7 cKO #1 represents one independent clone (n = 4) and TUT4/7 cKO #2 (n = 4) represents the other. P-values are denoted by p and are determined by Student's t-test. (E) Abundances of uridylated isomiRs of miR-200c-3p in DU145, IGROV1, and derived TUT4/7 cKOs from RNA-seq data analyzed with sRNAbench software (Barturen et al. 2014). (F) Down-regulation of miR-200c in TUT4/7 cKOs is accompanied by up-regulation of one of its targets, BCL2. (G) Each miRNA (in blue) is connected to mRNA targets that are implicated in cell migration or wound healing. Red dots denote mRNAs that are down-regulated, otherwise the mRNA is denoted in gray. This network was created using miRNet (Fan et al. 2016).
