TUT4/7 loss results in cancer cell-type-specific miRNA deregulation. (A) Western blot image of LIN28A in the stable cell line LIN28AOE DU145, DU145 (depicted as WT), and IGROV1 with α-tubulin (TBA4A) as a control (n = 2). Molecular mass of LIN28A is 26 kDa and of TBA4A is 50 kDa. (B) Principal component analysis of miRNA data set. PC1 versus PC2 in miRNA data for all genotypes. (C) Venn diagram showing gene overlap. (D,E) Violin plots displaying the expression trends in Clusters 0, 1, and 8 (D) and Clusters 4 and 12 (E). Fold change is calculated in the TUT4/7 double mutants (TUT4/7 cKO) relative to their respective wild-type control. (F,G) miRNA set enrichment analysis was performed with TAM 2.0 (Li et al. 2018). Enriched associations for the miRNA Cluster 1 and Cluster 8 with a similar deregulation pattern in TUT4/7 double mutants of IGROV1 (F) and for the miRNA Cluster 4 and Cluster 12 with a similar deregulation pattern in TUT4/7 double mutants of DU145 (G).
