Impact of TUT4/7 loss on proportion of terminally modified miRNA variants. (A) “A (any length)”, “C (any length)”, “G (any length)”, and “U (any length)” are nontemplated additions of A, C, G, or U, respectively, with length 1 (in the case of mono additions) to the longest chain of homopolymeric tail detected at the end of an miRNA. (B) The NTA types displayed are mono-A additions (A), di-A additions (AA), mono-A addition followed by a mono-U additions (AU), mono-U additions (U), mono-U additions followed by a mono-A additions (UA), and di-U additions (UU). (C) Mono-U additions in the wild-type control (WT; n = 4) and TUT4/7 double mutants (cKO; n = 8) of DU145 and IGROV1. µ cap denotes the mean and P-value is indicated by p. (D) Mono-U additions at 3p and 5p mature miRNAs in the wild-type control (WT; n = 4) and TUT4/7 double mutants (cKO; n = 8) of DU145 and IGROV1. µ cap denotes the mean and P-value is indicated by p. (E) Mono-A additions in the wild-type control (WT; n = 4) and TUT4/7 double mutants (cKO; n = 8) of DU145 and IGROV1. µ cap denotes the mean and P-value is indicated by p. (F) Venn diagram showing overlap between miRNAs that show an overall decrease in adenylation (↓A), miRNAs with a decrease in uridylation (↓U), miRNAs with an increase in uridylation (↑U), and miRNAs with an increase in adenylation (↑A).
