The landscape of translational stall sites in bacteria revealed by monosome and disome profiling

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FIGURE 3.
FIGURE 3.

Correspondence between identified pause sites and iNP. (A) Correlation between the molecular weight (M.W.) of polypeptidyl-tRNA predicted by gel migration in in vivo (left) and in vitro (right) iNP (Chadani et al. 2016) and that identified in disome profiling (Fig. 2A). Puromycin-resistant polypeptidyl-tRNAs in in vitro iNP are highlighted in blue. (B) The same as A but for the comparison with monosome pause sites (Fig. 2E).

This Article

  1. Published in Advance December 14, 2021, doi: 10.1261/rna.078188.120 RNA 28: 290-302

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