Logo of the RI positions around the TRS-putative sequences obtained from DI-tector. The conservation plotted as total height of the letters representing nucleotides is obtained as log2 (4)–Σn fi (n) log2 (fi (n)), where fi (n) is the frequency of nucleotide n in position i. Therefore a height equal to 2 corresponds to perfect conservation. The horizontal axis is the position with respect to the reference sequence (GISAID ID: EPI_ISL_414631). The green box highlights the canonical TRS. The alignment step to obtain this logo is described in the Materials and Methods section. Color code used: red for adenine and uracil, blue for cytosine and guanine.
