Canonical sgRNAs and some noncanonical sgRNAs are consistently observed across the three biological replicates. ORF names and numbers of counts of the 20 deletions with the most counts were observed in NGS data in three biological replicates. Data from different replicates have been normalized so that the number of viral reads observed in each replicate is constant (see Materials and Methods). Bar heights are given by the average of the three replicates (shown as white dots after normalization), and error bars represent the standard deviation. The colored bars below the ORF names indicate statistical significance of the count differences; ORFs above bars of different colors have statistically different junction counts (P-value ≤0.05, from a two-sample, two-tailed, Welch's unequal-variance t-test).
