Most of the DVG reads can be associated to canonical sgRNAs. Here we show the results of RNA-seq and alignment of the reads to the human and SARS-CoV-2 genomes. NGS library preparation was performed with a ribodepletion step. The unmapped reads have been further processed with DI-tector, and the resulting characterization of the DVG reads into deletions, insertions and copy-backs/snap-backs is given. The percentage of junction reads corresponding to canonical sgRNA that is standard annotated subgenomic ORFs for SARS-CoV-2 (S, 3A, E, M, 6, 7a, 7b, N, 10), is specified. All percentages are averaged over three biological replicates. Cells colors are given to classify reads in host-related reads (blue), viral reads (yellow), DVG reads (green), canonical sgRNA reads (red), other reads (gray).
