Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis

YicC and YloC have similar activities. RNase activity of E. coli YicC protein compared with B. subtilis YloC protein, on IR800-labeled RNA oligo. An amount of 30 pmol of RNA (labeled + unlabeled) was incubated at 37°C with 10 pmol of YicC or YloC protein for the times (in minutes) indicated above each lane. The number in parentheses is initial rate of product formation, relative to the initial rate for YloC (average of two experiments).