
RNase activity of YloC mutant proteins. An amount of 30 pmol RNA oligo (IR800-labeled + unlabeled) was incubated at 37°C with 10 pmol of wild-type or mutant YloC protein for the times (in minutes) indicated above each lane. Numbers in parentheses are the initial rate of product formation, relative to the initial rate for the wild type (average of three experiments).










