Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis

Nucleotide target specificity of YloC. (A) YloC (and YicC) cleavage pattern on [³²P]5′-end-labeled RNA oligos with different 26-nt sequences. The AUG sequence of each oligo is in bold. An amount of 30 pmol of RNA (labeled + unlabeled) was incubated with 10 pmol of YloC at 25°C for the indicated times (in minutes). (OH) RNA oligo treated with hydroxyl, (T1) T1 RNase digest. Migration of full-length (FL) RNA and T1 RNase digestion products indicated at the left. (B) YloC activity on polynucleotides containing a single base type indicated above each set of lanes. (C) Assay of YloC activity on [³²P]-labeled DHB1935 in the presence of an excess (1.5-fold or threefold) of unlabeled sense (S) or antisense (A) RNA. Lanes at right contained a [³²P]5′-end-labeled DNA oligo, with the same sequence as DHB1935, in the absence (−) and presence (+) of YloC.