Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing

mRNA scaffolds predict an unannotated ADGRE1 isoform. (A) GENCODE v32 annotations for ADGRE1 mRNA isoforms. (B) Nine high-confidence mRNA scaffolds for an unannotated ADGRE1 isoform. (C) Polyadenylation sites annotated by the Poly(A)Site 2.0 atlas (Herrmann et al. 2019). GM12878 specific data from: (D) Pol II ChIP-seq sites (Barski et al. 2007); (E) CAGE sites for the positive strand (Djebali et al. 2012); (F) three replicate DNase-HS tracks (Davis et al. 2018). (G) Read coverage from full-length nanopore 5′ RACE sequencing. (H) Human peripheral blood mononuclear cell (PBMC) nanopore cDNA reads (Volden et al. 2018). Red and blue lines indicated forward and reverse alignments, respectively.