Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing

mRNA scaffold 3′ end distance to known polyadenylation site. Reads with aligned 3′ ends upstream of a polyadenylation site (internal to the gene) are represented with negative distances, and downstream from a polyadenylation site (external to the gene) are represented with positive numbers. The dashed lines are at −60 and +10 from an annotated polyadenylation site, 99% of mRNA scaffolds fall within this range.