Identification of high-confidence human poly(A) RNA isoform scaffolds using nanopore sequencing

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FIGURE 2.
FIGURE 2.

Cap-adaptation performance on GM12878 RNA. Number of transcripts per gene counts per million (CPM) correlation plots (A) between untreated and treated samples, and (B) between full-length untreated and cap-adapted samples. Pearson's r (ρ) were 0.95 and 0.83, respectively. (C) Percent of RNA by class for untreated, unadapted, and cap-adapted reads. Unadapted refers to reads within the treated samples that are missing the adapter sequence. All class percentages are in Supplemental Table 3.

This Article

  1. RNA 28: 162-176