Distribution of footprint length for each strain and cycloheximide treatment condition. Fractions of each footprint length (nt) were calculated and averaged among replicates. Green and red lines indicate libraries prepared in the absence and presence of cycloheximide, respectively. Dashed and solid lines represent total and immunopurified (IP) ribosomes, respectively. Gray shaded areas highlight different classes of footprint size: from left to right, 20–23 nt = small (S), 27–32 nt = medium (M), and 37–43 nt = large (L). (A) Data from libraries prepared from two biological replicates of amino-terminally FLAG-tagged strains and their WT + EV control. (B) Data from libraries prepared from three biological replicates of carboxy-terminally FLAG-tagged strains and their WT + EV control.
