U6-II strains grow more slowly than WT and are more sensitive to tagging the Lsm2–8 and Sm rings. (A–D) Strains were grown in YPGal liquid media for 48 h at 30°C. Two technical replicates are shown as solid and dotted lines. Strains expressing WT U6, U6-II-Sm, and U6-II are separated by strain background based on the presence of (A) no epitope tagged proteins, (B) Lsm8-2xV5, and (C) Smd1-3xFLAG. (D) Calculated doubling times for strains shown in panels A–C. Plotted are the averages ± SD (bars) from four replicates (dots), except for U6-II/Lsm8-2xV5, which only has two technical replicates. (E) Yeast spotted onto YPGal solid media were incubated at the indicated temperatures and imaged on the indicated days.
