(A) Schematic for analysis of SNORD13 stability by RNase A/T1 assay. (B) Sequence of human SNORD13s analyzed. Antisense regions 18S-A, 18S-B, and 18S-C are highlighted in red, C and D-box motifs involved in k-turn formation in yellow, and putative stem regions in gray. In addition to wild-type (“WT,” black) sequence, disruptive mutations (“mut,” red), and rescue mutations (“comp,” red) were explored for stems I–IV. C and D box mutations were also analyzed (sequences provided in Supplemental Information). (C) Results of RNase A/T1 stability assay. Top box indicates protected RNA fragment corresponding to full length, transfected human versions of SNORD13. Bottom box indicates protected RNA fragments corresponding to endogenously expressed mouse SNORD13 background.
