HITS-CLIP analysis of human ALKBH8 reveals interactions with fully processed substrate tRNAs and with specific noncoding RNAs

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FIGURE 1.
FIGURE 1.

Wobble uridine tRNA modifications and ALKBH8 activities. (A) Known wobble uridine modifications in yeast. (B) The ALKBH8 reaction mechanism. The MTase domain of ALKBH8 together with the cofactor TRMT112 uses S-adenosyl-L-methionine (SAM) to convert cm5U into mcm5U. In the case of tRNAGly(UCC), the AlkB-like domain subsequently performs hydroxylation into (S)-mchm5U.

This Article

  1. Published in Advance October 3, 2022, doi: 10.1261/rna.079421.122 RNA 28: 1568-1581

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