Structures of RNA ligase RtcB in complexes with divalent cations and GTP

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FIGURE 3.
FIGURE 3.

Structures of PhoRtcB in complexes with GTP and manganese, cobalt, or nickel. Stereo views of the GTP•(Mn2+)2 (A), GTP•(Co2+)2 (B), and GTP•(Ni2+)2 complexes (C) are shown. Atomic contacts to GTP and metals are indicated by black dashed lines. The distance between the His404-Nε nucleophile and the GTP α-phosphorus is denoted by a magenta dashed line. Mn atoms (A), Co atoms (B), and Ni atoms (C) are rendered as magenta, cyan, and light green spheres, respectively. Anomalous difference peaks overlying the Mn and Co atoms (green mesh) and Ni atoms (blue mesh) are contoured at 3σ, 14σ, and 10σ, respectively.

This Article

  1. RNA 28: 1509-1518