Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing

RESC13 is not involved in editing initiation, while RESC14 has transcript-specific effects in initiation. (A) qRT-PCR analysis of RNA isolated from uninduced and RESC13 RNAi-induced samples using primer sets designed to detect pre-edited, fully edited and total RNA levels of A6 and COIII. Relative abundance of each transcript in induced versus uninduced RNAi cells is shown. Two biological replicates, each with three technical replicates, were performed. Replicate 1 RNA levels were normalized to 18S rRNA levels, and replicate 2 levels were normalized to Β-tubulin RNA levels. RESC13 target RNA levels are colored red. (B) Same as in A, but with RESC14 RNAi. RNA levels for both replicates were normalized to 18S rRNA levels. RESC14 target RNA levels are colored cyan. (C) The average number of normalized pre-edited A6 and COIII reads for uninduced samples and two RESC13 RNAi-induced samples determined through TREAT. Sequences were normalized to 100,000 reads. (D) Same as in C, but with two RESC14 RNAi-induced samples. (*) P_adj < 0.05 and (****) P_adj < 0.0001.