FIGURE 2.
Effect of poly(A) tail length on replication and translation. (A) GFP expression in BHK21J cells transfected with SINV replicon RNAs harboring different sizes of the poly(A) tail. Images show a representative area of a 48-well plate and are intentionally overexposed to show the density of cells present in this area of the plate. (B) Luciferase expression in BHK21J cells transfected with Renilla luciferase mRNAs harboring the 3′-UTR of SINV with the indicated length of the poly(A) tail. Error bars indicate standard deviation of two independent experiments.
