Identification of RS domains in human proteins. (A) The human proteome (UP000005640_9606; n = 20,600 proteins) was iteratively analyzed using LCD-Composer, each time using a unique combination of S composition threshold and R composition threshold. The heatmap indicates the number of proteins identified for each composition threshold criterium. (B) Proportion of identified proteins that are classified as RBPs by Gerstberger et al. (2014), contain an RRM, or are directly annotated with the GO term “RNA binding.” (C) Venn diagram showing the overlap between known SR/SR-related proteins (Long and Caceres 2009), putative SR/SR-related proteins (this study), RBPs (only considering RBPs in either the known or putative SR/SR-related protein groups), and RRM-containing proteins (as identified by Pfam). (D) GO term analysis of new SR-related RBPs identified in this study (proteins outlined in red in panel C).
