Argonaute binding within human nuclear RNA and its impact on alternative splicing

Effect of DROSHA knockout on genes identified as candidates from AGO2 eCLIP and RNA-seq of AGO knockout cells. Skipped exon events in DROSHA KO cells. (A) Semiquantitative PCR validation of skipped exon events in DROSHA KO cells. (B) Quantitation of data shown in part A. Error bars represent standard deviation (SD). (*) P < 0.05; (**) P < 0.01 compared with WT by t-test. (C) Scatter plot showing the exon inclusion/exclusion ratio between AGO1/2/3 KO and DROSHA KO. Each point represents average of three biological replicates. (Dashed line) 95% confidence interval. (Solid line) Best fit. RUBCN and FKBP14 were subject to further experimental validation (Fig. 7) and are highlighted in red.