Argonaute binding within human nuclear RNA and its impact on alternative splicing

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FIGURE 4.
FIGURE 4.

The effect of AGO knockouts on alternative splicing events. (A) Scheme showing various types of alternative splicing events. (B) Alternative splicing events observed upon AGO1, AGO2, AGO1/2, AGO1/2/3 knockout. (C) Alternative splicing events upon AGO1, AGO2, AGO1/2, AGO1/2/3 knockout associated with nearby AGO2 protein-binding sites detected by eCLIP within introns. (D) VENN diagrams showing the overlap in skipped exon events for the AGO1, AGO2, AGO1/2, and AGO1/2/3 knockout cell lines. (E) VENN diagrams showing the overlap of alternative splicing events in AGO1, AGO2, AGO1/2, and AGO1/2/3 knockout cell lines for genes that have intronic binding sites for AGO2 detect by eCLIP.

This Article

  1. Published in Advance June 9, 2021, doi: 10.1261/rna.078707.121 RNA 27: 991-1003

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