Perturbation of ribosomal subunit dynamics by inhibitors of tRNA translocation

Kan, Par, and HygB maintain the order of SSU body-head movements. (A) Par (dark salmon and lime) and HygB (green) binding sites on the SSU. SSU body and head are shown in blue and red, respectively, the LSU is gray. EF-G-dependent SSU body (light blue) and head rotation (orange) were monitored in the presence of Kan (B), Par (C), or HygB (D) and compared to the reactions in the absence of antibiotics (−AB; blue is SSU body; red is SSU head).