Vio inhibits formation of CHI state and all following events of translocation. (A) Vio binding sites on the SSU (pink and light green) and LSU (orange, magenta, and cyan) (Zhang et al. 2020). SSU body and head are highlighted in blue and red, respectively; LSU is gray. (B) EF-G-dependent SSU body rotation and head swiveling were monitored in the absence of antibiotic (−AB; blue and red, respectively) or in the presence of Vio (light blue and orange, respectively). Conformations of the SSU body and head domains (N, R, and S) are indicated. Here and in all subsequent experiments, PRE complexes assembled with double-labeled ribosomes (0.05 µM after mixing) were mixed with EF-G (4 µM) and GTP (0.5 mM).
