LINC01116 contributes to neuronal differentiation but does not affect cell cycle progression. (A) Representative immunofluorescence images of Control and RA SH-SY5Y cells, transfected with siRNA targeting LINC01116 and scrambled control, after staining for Tuj (βIII-tubulin) at day 3 post-differentiation (scale bar = 200 µm). White windows magnified in B. (C) Quantification of neurite length in Control and RA treated cells upon knockdown shows a significant reduction of neurite length in the differentiated cells upon LINC01116 knockdown (N = 3 biological replicates, n > 100 measurements, Student's t-test P < 0.05). (D) RT-qPCR of differentiation marker MOXD1 in Control and RA treated cells, transfected with siRNA targeting LINC01116 and scrambled control, shows significant reduction of MOXD1 expression in differentiated cells with reduced LINC01116 levels at day 3 post-differentiation (n = 3 biological replicates, Student's t-test P < 0.05).
