Translation of lncRNA-smORFs. (A) Workflow for identification of translated ORFs from Ribo-Seq and RNA-seq; see Materials and Methods for details. (B) Venn diagram of lncRNA-smORFs translated in Control and RA, with overlap. (C) Plots of translational efficiencies for protein-coding ORFs, lncRNA-smORFs, dORFS, and uORFs. (D) Length distribution of translated ORFs in lncRNAs, dORFs, and uORFs (in codons). (E) Poly-Ribo-Seq profile for LINC01116 in RA treatment. RNA-seq (Polysome) reads are gray and ribosome P sites are in purple, turquoise, and yellow according to frame. Purple lines mark beginning and end of translated smORF. All possible start and stop codons are indicated below. Framing within and outside translated smORF shown on left.
