Regulation of closely juxtaposed proto-oncogene c-fms and HMGXB3 gene expression by mRNA 3′ end polymorphism in breast cancer cells

HuR targets c-fms mRNA, not HMGXB3 mRNA. (A) HuR IP of BT20 cell lysates. Two-fold excess IgG was used for nonspecific binding. (B) HuR IP enriches c-fms mRNA and c-fms mRNA with the 3′ end extension (n = 3), (C) not HMGXB3 mRNA and HMGXB3 mRNA with the 3′ end extension (n = 3). (D) HuR overexpression decreases and shHuR increases HMGXB3 mRNA (n = 4). (E) HuR overexpression increases and shHuR decreases c-fms mRNA (n = 4). (F) Western analysis shows that HuR overexpression decreases and shHuR increases HMGXB3 protein. In contrast, HuR overexpression increases and shHuR decreases c-fms protein.