Regulation of closely juxtaposed proto-oncogene c-fms and HMGXB3 gene expression by mRNA 3′ end polymorphism in breast cancer cells

Luciferase reporter assay indicates that miR-324-5p has opposite effects on HMGXB3 and c-fms expression. (A,B) miR-324-5p mimic decreases and miR-324-5p inhibitor increases luciferase-HMGXB3 mRNA 3′ UTR reporter expression (n = 4). (C) miR-324-5p mimic decreases (black bar) and miR-324-5p inhibitor increases luciferase-HMGXB3 mRNA 3′ UTR reporter RNA half-life (red bar) (n = 3). (D,E) miR-324-5p mimic increases and miR-324-5p inhibitor decreases luciferase-c-fms mRNA 3′ UTR reporter expression (n = 4). (F) miR-324-5p mimic increases (black bar) and miR-324-5p inhibitor decreases luciferase-c-fms mRNA 3′ UTR reporter RNA half-life (red bar) (n = 3).