Regulation of closely juxtaposed proto-oncogene c-fms and HMGXB3 gene expression by mRNA 3′ end polymorphism in breast cancer cells

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FIGURE 4.
FIGURE 4.

miR-324-5p regulates c-fms mRNA expression thru HMGXB3 mRNA. (A) miR-324-5p mimic decreases HMGXB3 mRNA and increases c-fms mRNA (n = 4). (B) Western analysis shows increased expression of c-fms protein and decreased expression of HMGXB3 protein by miR-324-5p mimic. Numbers below bands indicate band intensities scanned by ImageJ. (C) miR-324-5p mimic increases c-fms mRNA half-life (n = 3). (D) miR-324-5p mimic decreases HMGXB3 mRNA half-life (n = 3). (E) miR-324-5p inhibitor increases HMGXB3 mRNA and decreases c-fms mRNA (n = 4). (F) Western analysis shows decreased expression of c-fms protein and increased expression of HMGXB3 protein by miR-324-5p inhibitor. Numbers below bands indicate band intensities scanned by ImageJ. (G) miR-324-5p inhibitor decreases c-fms mRNA half-life (n = 3). (H) miR-324-5p inhibitor increases HMGXB3 mRNA half-life (n = 3).

This Article

  1. Published in Advance June 21, 2021, doi: 10.1261/rna.078749.121 RNA 27: 1068-1081

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