Mapping and quantification of transcripts with the 3′ end extensions. (A) Mapping of 3′ end extension by RT-PCR. The HMGXB3 mRNA with the 3′ end extension was reverse transcribed by 10 primers derived from the c-fms mRNA sequence. RT transcript was PCR amplified for mapping. The c-fms mRNA with the 3′ end extension was reverse transcribed by eight primers derived from the HMGXB3 mRNA sequence. RT transcript was PCR amplified for mapping. A detailed mapping strategy is described in Supplemental Figure S2. (B) qRT-PCR indicates that c-fms mRNA with 3′ end extension is 4.1% of total c-fms mRNA (n = 4). (C) qRT-PCR indicates that HMGXB3 mRNA with 3′ end extension is 1.1% of total HMGXB3 mRNA (n = 4). Detailed quantification strategy is described in Supplemental Figure S2.
