Regulation of closely juxtaposed proto-oncogene c-fms and HMGXB3 gene expression by mRNA 3′ end polymorphism in breast cancer cells

c-fms and HMGXB3 mRNA 3′ end polymorphism. (A) Both HMGXB3 gene and c-fms gene are closely juxtaposed in chromosome 5. The intergenic region is 162 bp. ³²P-labeled strand-specific antisense RNA probe 1 derived from the HMGXB3 mRNA coding region detects HMGXB3 mRNA. ³²P-labeled strand-specific antisense RNA probe 2 derived from the 3′ end of c-fms mRNA detects 3′ end extended HMGXB3 mRNA. ³²P-labeled strand-specific antisense RNA probe 3 derived from c-fms mRNA coding region detects c-fms mRNA. ³²P-labeled strand-specific antisense RNA probe 4 derived from the 3′ end of HMGXB3 mRNA detects 3′ end extended c-fms mRNA. Detailed description of probe generation is in Supplemental Figure S1. (B) Northern blot shows HMGXB3 mRNA and HMGXB3 mRNA with 3′ end extension. HMGXB3 mRNA is detected in 1 h exposure. In contrast, HMGXB3 mRNA with 3′ end extension is detected in 96 h exposure. (C) Northern blot shows c-fms mRNA and c-fms mRNA with 3′ end extension. C-fms mRNA is detected in 1 h exposure. In contrast, c-fms mRNA with 3′ end extension is detected in 96 h exposure.