Fusion protein EWS-FLI1 is incorporated into a protein granule in cells

EWS-FLI1 can alter effects on cell growth for an EWSR1 knockdown. (A, left) Growth on soft agar was assessed for HEK293T/17 cells after transfection of empty plasmids or a plasmid expressing V5-EWS-FLI1. (Right) Colonies were counted to reveal no change in averaged values relative to siSCR treatment (n = 4). (B, left) Soft agar assays were performed with HEK293T/17 cells expressing V5-EWS-FLI1 and cotransfected with siSCR or siEWSR1 (n = 6). (Right) Colonies were counted to reveal a reduction in averaged values relative to siSCR treatment. Error bars represent standard deviation. Student's t-test was calculated for assuming equal variances: (***) P < 0.001.