Loss of EWSR1 inhibits anchorage-independent growth in Ewing sarcoma cells. (A, left) Western assays reveal knockdown for EWSR1 or EWS-FLI1 in A673 cells for treatment with siSCR, siEF, or siEWSR1. (Right) Averaged levels of protein were determined by densitometry relative to siSCR treatments (n = 3). (B, left) Soft agar assays were performed with A673 cells treated with siSCR, siEF, or siEWSR1. (Right) Colonies were counted to reveal reductions in averaged values relative to siSCR treatment (n = 3). (C, left) Western assays reveal knockdown for EWSR1 or EWS-FLI1 in HEK293T/17 cells for treatment with siSCR, siEF, or siEWSR1. (Right) Averaged levels of protein were determined by densitometry relative to siSCR treatments (n = 4). (D, left) Soft agar assays were performed for HEK293T/17 cells treated with siSCR, siEF, or siEWSR1. (Right) Colonies were counted to reveal no change in averaged values relative to siSCR treatment (n = 4). All error bars represent standard deviation. Student's t-test was calculated assuming equal variances: (**) P < 0.01; (*) P < 0.05; n.s., not significant (P > 0.05).
