CRED9: a differentially expressed elncRNA regulates expression of transcription factor CEBPA

(A) CRED9 and (B) CEBPA mRNA expression in HeLa cells following cotransfection of dCas9-VPR expression plasmid and either sgRNA expression plasmids targeting upstream of CRED9 TSS or the promoter of CEBPA. (C) CRED9 and (D) CEBPA mRNA expression in HepG2 or HeLa cells following transfection of 30 nM CEBPA short activating RNA. In both experiments, increased expression of one transcript correlated with increased expression of the other. (n = 3 biological replicates; normalized to GAPDH mRNA; error bars: std; P-values from unpaired t-tests where significance “*” cut-off is P ≤ 0.05, [**] P ≤ 0.01, [***] P ≤ 0.001, [****] P ≤ 0.0001, [ns] not significant).