Knockdown of CRED9 by 200 nM ASO gapmer 4 causes a reduction in CEBPA protein expression. Detection by fluorescent western blot: (A) Lanes, 1: CtrlA, 2: Gap4, 3: CtrlA, 4: Gap4, 5: HeLa lysate, 6: CtrlA, 7: Gap4, 8: CtrlA, 9: Gap4. Upper red arrow: CEBPA 42 kDa isoform. Lower red arrow: CEBPA 30 kDa isoform. Hep3B: 42 kDa (FC: −2.20, CV: 6.33%) 30 kDa (FC: −3.18, CV: 6.21%). HepG2: 42 kDa (FC: −1.52, CV: 14.44%) 30 kDa (FC: −1.43, CV: 30.12%). Reduced expression of CEBPA in Hep3B is robust for both isoforms. Knockdown in HepG2 is more subtle, likely due to less efficient transfection. (B) Revert 700 total protein stain of the membrane from A used for total protein normalization. (n = 2 biological replicates, [FC] fold change, [CV] coefficient of variation).
