IsoSplitter: identification and characterization of alternative splicing sites without a reference genome

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FIGURE 4.
FIGURE 4.

The comparisons of prediction results using PASA, AStrap, and IsoSplitter. (A) The majority of AS events were identified by PASA; IsoSplitter predicted more AS events than Astrap. (B) Evaluation of missed AS events by IsoSplitter compared to PASA. (C) An example of missed AS event of retained intron by IsoSplitter. The two AS isoforms (NM_001340557.1 and NM_001036510.3) are from the gene model AT4G05590 in Arabidopsis thaliana. The transcripts are not supported by two consecutive aligned regions and hence were missed in the IsoSplitter prediction.

This Article

  1. RNA 27: 868-875