Overview of the µIVC-Useq pipeline. The microfluidic-assisted in vitro compartmentalization (μIVC) step is made of three main steps during which: (i) The genes contained in a library are individualized prior to being amplified, (ii) droplets containing amplified genes are fused one-to-one with an in vitro expression mixture supplemented in fluorogen, and (iii) the fluorescence of each droplet is measured and used to sort them accordingly. In µIVC-Useq, the screening step is followed by a next-generation sequencing (NGS) analysis of the sequences contained in enriched libraries while an unsupervised bioinformatic pipeline allows the rapid identification of molecules of interest.
