FIGURE 4.
Methylation does not protect an Ago1-loaded miRNA from TDD. (A) Decay of methylated (2′-O-Me) or nonmethylated (2′-OH) miR-7 transfected into wild-type and dora cells. Decay was monitored on a northern blot probed both for miR-7 and endogenous miR-11, which served as a loading control. (B) As in A, but in the nibbler background. (C) Quantification of the results in B and those of one additional biological replicate, which used independent clonal cell lines (Supplemental Fig. S4E). The lines show the best least-squares fits of an exponential-decay model. Half-lives, indicated in parentheses, encapsulated the rates of both dilution and decay.
