Protein expression from trans-spliced mRNA products. (A) Schematic representation of circR/G.PGK and circG/R.PGK expression vectors. (B) Schematic diagram illustrating how trans-splicing of the indicated cotransfected vectors can lead to the production of full-length RLuc and GFP proteins. Due to space constraints, not all possible trans-splicing reactions are shown. (C) Luciferase values obtained from 293T cells cotransfected with the indicated expression vectors. RLuc RLU values were normalized to total protein levels in the extracts. n = 4 ± SD. (D) Flow cytometry showing representative GFP expression obtained from 293T cells transfected with the indicated individual or combinations of plasmids.
