Analysis of GTP addition in the reverse (3′–5′) direction by human tRNAHis guanylyltransferase

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FIGURE 2.
FIGURE 2.

Addition activities of wild-type NTP and GTP analogs by HsThg1. (A) Natural NTPs and GTP analogs used in the nucleotide addition reaction. (B,C) The relative kobs values of nucleotide addition reactions with various GTP analogs (0.1 mM) for the template Y = A (B), Y = C (C). Values are relative to the kobs value of wild-type activity (GTP addition in Y = A). kobs values of HsThg1 (this study) and CaThg1 (Nakamura et al. 2018b) are colored black and gray, respectively. The bars in the graphs are SD of more than two independent experiments.

This Article

  1. Published in Advance March 23, 2021, doi: 10.1261/rna.078287.120 RNA 27: 665-675

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