An expanded class of histidine-accepting viral tRNA-like structures

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FIGURE 2.
FIGURE 2.

Histidine-accepting tRNA-like structures adopt a conserved secondary structure. Chemical probing of four representative TLSHis RNAs using the SHAPE reagent NMIA: (A) Tobacco Mosaic virus, (B) Ribgrass Mosaic virus, (C) Hibiscus Latent Fort Pierce virus, and (D) Diascia Yellow Mottle virus. Reactivity was background subtracted and normalized to flanking 5′ and 3′ normalization hairpins (not depicted; see Supplemental File 2 for sequence details). Coloring represents degree of normalized modification according to the inset legend.

This Article

  1. Published in Advance April 2, 2021, doi: 10.1261/rna.078550.120 RNA 27: 653-664

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