Comparison of tRNA quantification in yeast using OTTER and northern blotting. (A) The upper panel shows northern blotting analyses of tRNA-Leu isoacceptors in yeast cells grown in YPD or YPGly. Three replicates (0.50 µg of total RNA per lane) were hybridized with the probe shown on the top. The sequences of the probes are shown in Supplemental Table S1. The lower panel shows a quantification analysis of the northern blotting and a comparison of the tRNA-Leu isoacceptor amounts obtained from northern blotting and OTTER. (B) Changes in the amounts of the indicated tRNA-Leu isoacceptors in the YPGly-grown cells normalized by those in the YPD-grown cells. Error bar: standard deviation.
