OTTER, a new method for quantifying absolute amounts of tRNAs

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FIGURE 3.
FIGURE 3.

The tRNA repertoires of yeast cells grown in the rich glucose medium. (A) The absolute amounts of tRNAs in RNA samples extracted from yeast cells growing to the log phase (circles) or stationary phase (triangles) were measured by OTTER. Each data set represents the average of three biological replicates, and the error bars indicate the standard deviations. The tRNA gene copy numbers were obtained from Genomic tRNA Database (GtRNAdb) (Chan and Lowe 2016). (B) Fold-changes in tRNA abundances between the stationary phase and log phase cells. The tRNA species with the smallest change (tRNA-ArgCCG) and the largest change (tRNA-GlyCCC) are indicated.

This Article

  1. RNA 27: 628-640