RNA aptamer gradients with Peach I and MIII A10U in the presence of 100 nM TO1-B and 100 nM TO3-B. Fluorescent signals from singly titrated aptamers for (A) Peach I and (B) MIII A10U as well as from simultaneous aptamer gradients with (C) increasing Peach I and decreasing MIII A10U (D) for both increasing Peach I and MIII A10U over a range of 0 to 50 nM RNA. Fits were weighted with triplicate readings of simultaneous gradients (C,D) and a singlet reading of single aptamer gradients (A,B). (E) In a blinded study, the correlation between the actual and inferred aptamer concentrations shown in Table 4 is plotted. Concentrations of Peach I (red) and MIII A10U (green) in samples A to F were quantified from fits in A–D and Table 3.
