FIGURE 4.
Melting curves of Peach and Mango Aptamers (200 nM) in the presence of excess (2 µM) TO1-B (left) or TO3-B (right). (A) Peach I, (B) Peach II, and (C) MIII A10U. Simple derivatives of each melting curve are plotted underneath each fluorescent melting curve. Darker colors represent data from increasing temperature ramps, while lighter colors represent data from deceasing temperature ramps. A ramp rate of 1°C/min starting at 90°C and moving to 20°C, and returning to 90°C was used.
