RNA Peach and Mango: orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands

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FIGURE 3.
FIGURE 3.

Relative and absolute fluorescence of the Peach and Mango aptamers. Aptamers from the Mango and Peach series (20 nM each) were incubated with 100 nM TO1-B and 200 nM TO3-B. Green dots indicate total fluorescence in the TO1-B channel (RFU535), while red dots indicate total fluorescence in the TO3-B channel (RFU658). Unbound TO1-B and TO3-B brightness is indicated by “Free.”

This Article

  1. Published in Advance March 5, 2021, doi: 10.1261/rna.078493.120 RNA 27: 604-615

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