RNA Peach and Mango: orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands

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FIGURE 2.
FIGURE 2.

Competitive in vitro selection for TO3-B aptamers. (A) Chemical structures of the competitors in the TO3-B aptamer selection. (B) General scheme of the conditions for the 16 round TO3-B selection with indicated competitor molecules shown by the horizontal bars. Explicit competitor molecule details are found in Supplemental Table 1. The left axis depicts incubation times for preincubation, RNA binding, and wash 1 and 2 steps as indicated by the legend. The right axis indicates in red the competitor molecule concentration.

This Article

  1. RNA 27: 604-615