Genetic interactions between Ecm2 and U6 or Prp8 first- and second-step alleles. (A) Illustration of how alleles of Prp8, Prp16, and U6 function to promote the first or second step of splicing. (B) A first- or second-step allele of U6 (U57C or U57A, respectively) was combined with URA3 plasmids either lacking or coding for Ecm2 variants in ecm2Δ yeast. The strains were then tested for suppression or exacerbation of temperature-dependent growth phenotypes. Yeast were plated on -URA dropout media and imaged after 2 days of growth. (C) First- and second-step alleles of Prp8 (red and green, respectively) were combined with ecm2Δ and tested for suppression or exacerbation of temperature-dependent growth phenotypes. Yeast were plated on YPD media and imaged after 3 (23°C, 30°C, or 37°C) or 10 (16°C) days of growth.
