Saccharomyces cerevisiae Ecm2 modulates the catalytic steps of pre-mRNA splicing

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FIGURE 3.
FIGURE 3.

Genetic interactions between Ecm2 and U2 stem II mutations. (A) Schematic of stem IIa/IIc toggling. Mutations which disfavor stem IIc (U2-2,4 and ΔCC; green-colored labels) are shown in the stem IIa structure. Mutations which disfavor stem IIa (G53A, IIc+; red-colored labels) are shown in the stem IIc structure. Nucleotides that are deleted in the ΔIIb mutant are shown in purple. (B) Mutations in stem II were combined with ecm2Δ and tested for suppression or exacerbation of temperature-dependent growth phenotypes. Yeast were plated on YPD media and imaged after 3 (23°C, 30°C, or 37°C) or 10 (16°C) days of growth.

This Article

  1. Published in Advance February 5, 2021, doi: 10.1261/rna.077727.120 RNA 27: 591-603

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